Chemical found in widely used sweetener breaks up DNA

A new study finds a chemical formed when we digest a widely used sweetener is "genotoxic," meaning it breaks up DNA. The chemical is also found in trace amounts in the sweetener itself, and the finding raises questions about how the sweetener may contribute to health problems.

At issue is sucralose, a widely used artificial sweetener. Previous work by the same research team established that several fat-soluble compounds are produced in the gut after sucralose ingestion. One of these compounds is sucralose-6-acetate.

Our new work establishes that sucralose-6-acetate is genotoxic. We also found that trace amounts of sucralose-6-acetate can be found in off-the-shelf sucralose, even before it is consumed and metabolized."

Susan Schiffman, corresponding author of the study and adjunct professor in the joint department of biomedical engineering at North Carolina State University and the University of North Carolina at Chapel Hill

"To put this in context, the European Food Safety Authority has a threshold of toxicological concern for all genotoxic substances of 0.15 micrograms per person per day," Schiffman says. "Our work suggests that the trace amounts of sucralose-6-acetate in a single, daily sucralose-sweetened drink exceed that threshold. And that's not even accounting for the amount of sucralose-6-acetate produced as metabolites after people consume sucralose."

For the study, researchers conducted a series of in vitro experiments exposing human blood cells to sucralose-6-acetate and monitoring for markers of genotoxicity.

"In short, we found that sucralose-6-acetate is genotoxic, and that it effectively broke up DNA in cells that were exposed to the chemical," Schiffman says.

The researchers also conducted in vitro tests that exposed human gut tissues to sucralose-6-acetate.

"Other studies have found that sucralose can adversely affect gut health, so we wanted to see what might be happening there," Schiffman says. "When we exposed sucralose and sucralose-6-acetate to gut epithelial tissues – the tissue that lines your gut wall – we found that both chemicals cause 'leaky gut.' Basically, they make the wall of the gut more permeable. The chemicals damage the 'tight junctions,' or interfaces, where cells in the gut wall connect to each other.

"A leaky gut is problematic, because it means that things that would normally be flushed out of the body in feces are instead leaking out of the gut and being absorbed into the bloodstream."

The researchers also looked at the genetic activity of the gut cells to see how they responded to the presence of sucralose-6-acetate.

"We found that gut cells exposed to sucralose-6-acetate had increased activity in genes related to oxidative stress, inflammation and carcinogenicity," Schiffman says.

"This work raises a host of concerns about the potential health effects associated with sucralose and its metabolites. It's time to revisit the safety and regulatory status of sucralose, because the evidence is mounting that it carries significant risks. If nothing else, I encourage people to avoid products containing sucralose. It's something you should not be eating."

Journal reference:
  • Schiffman, S. S., et al. (2023) Toxicological and pharmacokinetic properties of sucralose-6-acetate and its parent sucralose: in vitro screening assays. Journal of Toxicology and Environmental Health Part B.


  1. Brandon Alloe Brandon Alloe United States says:

    You can toss out your studies if you look at my body. I have used Splenda several year. No leaky gut issues. I think this leaky gut thing is a bunch of ...

  2. Michele Green Michele Green United States says:

    So you tested this on unborn babies?  What happened to the babies once your experiment was over?

  3. Tina Simmons Tina Simmons United States says:

    R U kidding me don't eat this?! How about DONT SELL IT TO US CUZ KIDS ARE EATING TOXIC STUFF ALL DAY LONG CUZ U KEEP SELLING IT TO THEM LIKE RITZ, POP TARTS, SKITTLES, U NAME IT!!! Leaders in this country disgust me & frankly I'm shocked that in order to eat healthy & make sure I don't ingest toxic things from the food they sell, I gotta move to Europe! It's not far off from a 3rd world country no?!! Camt drink water in Mexico & can't eat anything in America or it'll kill you! Big biz & the leadership along w/the govt are ruining this entire country!! Morals should ALWAYS BE THE LAW FOR ANY COUNTRY NOT MONEY!! We still allow animal testing in places big biz sells tlegere it's legal like China! L'Oreal is one of em & it sickens me! I thot SeaWorld shut down the whale exhibit long ago but nope not even close. This HAS TO STOP WAKE UP PPL!! Every other popular beauty product Americans use haas known carcinogens in it thus European leaders remove it from the market. What does America's leaders do? NOTHING KEEP SELLING IT TO MAINLY KIDS NO LESS! Then they launch some over weight or obese kids marketing campaign to make u think they care just like the recent vape campain. They did that cuz cigs is a HIGE BIZ not cuz they care one bit about kids vaping or kids being over weight & dying from complications around it. I'm so tired of this country! Get a clue stop buying the crap & going to shows where it don't take aa genius to figure out the whales are being held captive in aaan inhumane enclosure! Enough Already !!!!!! 🤬☠️👎

  4. Brandon Alloe Brandon Alloe United States says:

    I disagree. First of all, there is no such thing as leaky gut. Second, if this alters DNA maybe that is why some people are immune to COVID. Third, the only difference in Sucrose and Sucralose is that one oxygen atom is replaced by a chlorine atom. That chemical compound theoretically should appear in tap water. These scientists need to lay of cocaine.

  5. Major Havoc Major Havoc United States says:

    Who could of guessed man-made chemicles that mimic sugar, could be bad for you?

  6. Patrick Harris Patrick Harris United States says:

    Sugar sales must be down.

The opinions expressed here are the views of the writer and do not necessarily reflect the views and opinions of News Medical.
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